Page 16 - M.Sc-Chemistry

SASTRA Deemed to be University M.Sc. (Chemistry)

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Course Code: CHY411
Semester: I

ANALYTICAL CHEMISTRY

Course Objectives:
This course aims to:
 Help the learners to understand the different analytical tools used for determining the
purity, stability and separation of mixtures
 Train the learners in handling different analytical data with their error significance

UNIT – I 18 Hours
DATA HANDLING AND STOICHIOMETRIC CALCULATIONS
Accuracy and precision, significant figures, multiplication and division, addition and
subtraction, logarithms. Determinate errors, Indeterminate errors. Ways of expressing
accuracy: absolute errors, relative error. Standard deviation. Confidence limit. Tests of
significance: F test, Student t test, Paired t test, t Test with multiple samples. Rejection of a
result: the Q test. Median Vs. mean, Range Vs. standard deviation, Confidence limits using
the range. Linear least squares. Correlation coefficient. ANOVA.

Stoichiometric calculations: Atomic, molecular and formula weights, Daltons, moles, molarity,
normality, molality. Expressions of analytical results: Weight/weight basis, Solid samples-%,
ppt, ppm, ppb, mg%; Liquid samples: Weight/volume basis, %, ppt, ppm, ppb, meq.
Expressing concentrations as equivalents of substances.

UNIT – II 15 Hours
SEPARATION TECHNIQUES
Paper chromatography, thin layer chromatography, general theory of column
chromatography, chromatographic resolution, capacity factor, column selectivity, column
efficiency, peak capacity, Non-ideal behavior. Optimization of chromatographic separations:
using capacity factor to optimize resolution, using column selectivity to optimize resolution:
multiple paths, longitudinal diffusion, mass transfer, putting it all together. Gas
chromatography, HPLC, ion-exchange chromatography, size exclusion chromatography,
super critical fluid chromatography.

Electrophoresis: electrophoretic mobility, electro-osmotic mobility, instrumentation, capillary
electrophoresis methods, isoelectric focusing. Atomic Absorption Spectroscopy.

UNIT – III 15 Hours
ELECTROCHEMICAL METHODS OF ANALYSIS
Coulometric methods of analysis: controlled-potential coulometry, controlled-current
coulometry. Voltammetric methods of analysis: voltammetric measurements, current in
voltammetry, shape of voltammograms, quantitative and qualitative aspects of voltammetry.
Voltammetric techniques: polarography, hydrodynamic voltammetry, stripping voltammetry,
amperometry, applications. Electrogravimetry, impedance spectroscopy

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